Responsive image

 首頁 |   Language  : English


健康科學學院 > 醫學研究所 > 期刊論文 >

題名:Evaluation of microvasculature at the auditory midbrain – the benefits of sectioning at a tangential angle
作者:Edmund Cheung So, Hui-Pin Lu, Chung Hsi Hsing, Sheng Nan Wu, Yu-Kai Chang, Paul W.F. Poon
期刊名稱: Microscopy research and technique
摘要:Vascular remodeling in the brain occurs as a plastic change following neural over-activity. The auditory midbrain (or inferior colliculus, IC) is an ideal place to study sound induced vascular changes because it is the brain’s most vascularized structure and it is tonotopically organized. However, its micro-vascular pattern remains poorly understood. Since the IC is a sphere-like structure, the histological assessment of vasculature could depend on the angle of sectioning. Here, we studied the effects of cutting the IC at different angles on microvascular assessment, specifically: micro-vascular density and the shape of microvascular lumen. Photomicrographs were taken from 5 mm toluidine blue-stained histological sections obtained at two angles of sectioning: (a) the conventional coronal sectioning, and (b) a novel “tangential” sectioning (tangential to the dorso-medial surface of the IC). Results showed that the tangential sections, in comparison with the coronal sections, yielded (a) a higher count of micro-vascular density and (b) a higher proportion of round-shaped micro-vascular lumens. This discrepancy in results between two cut angles is likely related to the spatial pattern of blood vessels supplying the IC. We propose that the tangential sectioning should be adopted as standard for the accurate study of microvasculature in the IC.
英文關鍵字:inferior colliculus; functional capillaries; histology sectioning
DOI: 10.1002/jemt.22450