|題名：||Cordycepin Inhibits Human Gestational Choriocarcinoma Cell Growth by Disrupting Centrosome Homeostasis|
|作者：||Chia-Yih Wang , Shih-Wei Tsai, Han-Hsiang Chien, Ting-Yu Chen,, Shi-Yuan Sheu, Edmund Cheung So , Bu-Miin Huang|
|期刊名稱：||Drug Design, Development and Therapy|
|摘要：||Introduction: Human gestational choriocarcinoma, a type of gestational trophoblastic
disease, occurs after miscarriage, abortion, ectopic pregnancy, or molar pregnancy. Despite
recent advances in the mechanism of anticancer drugs that induce human gestational
choriocarcinoma apoptosis or block its growth, new therapeutic approaches are needed to
be established. Cordycepin is an active anti-cancer component extracted from Cordyceps
sinensis. It prevents cell proliferation both in vitro and in vivo.
Materials and Methods: Here, we examined cell growth by counting cell numbers, and
performing a flow cytometry assay and EdU incorporation assay. Centrosome and cytoskeleton-related structures were observed by immunofluorescence assay. The DNA damagerelated signaling was examined by Western blot assay.
Results: Here, we showed that cordycepin inhibited human gestational choriocarcinoma cell
proliferation and induced cell death. In addition, treatment with cordycepin activated DNAPK and ERK, thus inducing centrosome amplification and aberrant mitosis. These amplified
centrosomes also disrupted microtubule arrays and actin networks, thus leading to defective
cell adhesion. Furthermore, cordycepin induced autophagy for triggering cell death.
Conclusion: Thus, our study demonstrates that cordycepin inhibits cell proliferation and
disrupts the cytoskeleton by triggering centrosome amplification.
Keywords: human gestational choriocarcinoma, cordycepin, centrosome, cell growth,
|英文關鍵字：||human gestational choriocarcinoma, cordycepin, centrosome, cell growth|