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題名:Cordycepin Inhibits Human Gestational Choriocarcinoma Cell Growth by Disrupting Centrosome Homeostasis
作者:Chia-Yih Wang , Shih-Wei Tsai, Han-Hsiang Chien, Ting-Yu Chen,, Shi-Yuan Sheu, Edmund Cheung So , Bu-Miin Huang
期刊名稱:Drug Design, Development and Therapy
發表頁數:pp 2987–3000
發表年份:2020
發表月份:7
摘要:Introduction: Human gestational choriocarcinoma, a type of gestational trophoblastic disease, occurs after miscarriage, abortion, ectopic pregnancy, or molar pregnancy. Despite recent advances in the mechanism of anticancer drugs that induce human gestational choriocarcinoma apoptosis or block its growth, new therapeutic approaches are needed to be established. Cordycepin is an active anti-cancer component extracted from Cordyceps sinensis. It prevents cell proliferation both in vitro and in vivo. Materials and Methods: Here, we examined cell growth by counting cell numbers, and performing a flow cytometry assay and EdU incorporation assay. Centrosome and cytoskeleton-related structures were observed by immunofluorescence assay. The DNA damagerelated signaling was examined by Western blot assay. Results: Here, we showed that cordycepin inhibited human gestational choriocarcinoma cell proliferation and induced cell death. In addition, treatment with cordycepin activated DNAPK and ERK, thus inducing centrosome amplification and aberrant mitosis. These amplified centrosomes also disrupted microtubule arrays and actin networks, thus leading to defective cell adhesion. Furthermore, cordycepin induced autophagy for triggering cell death. Conclusion: Thus, our study demonstrates that cordycepin inhibits cell proliferation and disrupts the cytoskeleton by triggering centrosome amplification. Keywords: human gestational choriocarcinoma, cordycepin, centrosome, cell growth, microtubule
關鍵字:人類絨毛膜癌
英文關鍵字:human gestational choriocarcinoma, cordycepin, centrosome, cell growth
DOI: https://doi.org/10.2147/DDDT.S252401
Vol: 2020
NO:14
ISSN:11778881